Ovarian Club X and CoGEN in Asia

SpeakerS

Katsuhiko HAYASHI (Japan)

The female germ line undergoes a unique sequence of differentiation processes that finally endows the eggs with totipotency. The reconstitution in vitro of oogenesis using pluripotent stem cells, which eventually produces functional oocytes, has long been sought in reproductive biology and developmental biology, since it would contribute not only to a better understanding of the basic mechanisms underlying totipotency, but also to an alternative source of gametes for animal reproduction.

We recently developed a culture system that reconstitutes the entire process of oogenesis from mouse pluripotent stem cells, yielding in vitro-generated eggs that gave rise to healthy offspring. In the symposium, I will introduce recent advances in the in vitro culture system with the relevant transcriptome data and update current experiments to address molecular mechanisms underlying oogenesis. I hope to have an opportunity to discuss the data, so that we can find mutually beneficial collaborations.  

Biography
Katsuhiko Hayashi, Ph.D. (born Dec 2, 1971)
Full professor, Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University.

1994-1996, MS course of Meiji University,
1996-2002, Assistant professor in Tokyo University of Science,
2002-2005, Staff researcher in Osaka Medical Center,
(2004) PhD in Tokyo University of Science,
2005-2009, Post-doctoral fellow in the Gurdon institute, University of Cambridge,
2009-2014, Associate professor in Graduate School of Medicine, Kyoto University,  
2014- Present Full professor in Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University.

Research interest
Hayashi has been working on basic reproductive biology to understand how germ cell differentiation is genetically and epigenetically regulated. Especially, Hayashi focuses on mechanisms underlying differentiation of primordial germ cells and oocytes. A feature of the research is to develop an original culture system that reproduces germ cell differentiation. By using the culture system, Hayashi elucidates the basic mechanisms and produce a high quality of primordial germ cells and eggs that are capable of full-term development.  

 

Abstract

Development of a model culture system to produce eggs in vitro

The female germline undergoes a unique sequence of differentiation processes that finally endows the eggs with totipotency. The reconstitution in vitro of oogenesis using pluripotent stem cells, which eventually produces functional oocytes, has long been sought in reproductive biology and developmental biology, since it would contribute not only to a better understanding of the basic mechanisms underlying totipotency, but also to an alternative source of gametes for reproduction.

We recently developed a culture system that reconstitutes the entire process of oogenesis from mouse pluripotent stem cells, yielding in vitro-generated eggs that gave rise to healthy offspring. In the symposium, I will introduce recent advances in oocyte production from pluripotent stem cells with the relevant transcriptome data and update current experiments to address molecular mechanisms underlying oogenesis. I hope that we have an opportunity to discuss the data, so that we can find mutually beneficial collaborations.